While analysis of the genome or proteome can be predictive of the fate of an organism, its metabolome is informative of the outcome of events on an organism. The metabolome can thus be regarded as closer to the actual phenotype than either the genome or proteome. Lipids represent a major component of the metabolome and their hydrophobic and amphipathic nature dictates their separate analysis from more water- soluble metabolites; hence, the discipline of lipidomics has emerged. In this short chapter, we will highlight the different strategies in lipidomics which have now reached maturity, i.e., shotgun and chromatography-based mass spectrometry approaches. We will discuss some of the newer technologies coming to the forefront e.g., the use of derivatization chemistry, and comment on exciting developments now being made in the lipidomic feld, e.g., surface analysis and lipid imaging. Finally, we will comment on some of the dangers encountered using an “omics” approach in biochemical analysis.