توضیحات
ABSTRACT
Lutzomyia longipalpis is the main vector of visceral leishmaniasis (VL) in America. Physiological and molecular mechanisms of Leishmania infection in sand fies have been studied during the frst gonotrophic cycle. Tere are few studies about these interactions during the second gonotrophic cycle mainly because of the difculties maintaining sand fies through sequential feeds. Here we standardized conditions to perform the second blood feed efciently, and our results show that oviposition is an essential factor for the success of multiple feeds. We evaluated the impact of the second blood meal on longevity, protein digestion, trypsin activity, and Leishmania mexicana development within L. longipalpis gut. Mortality of blood-fed females increases afer second blood meal as compared to sugar-fed females. Trypsin activity was lower during the second gonotrophic cycle. However, no diference in protein intake was observed between blood meals. Tere was no diference in the population size of Leishmania in the gut afer both blood meals. In this work, we presented an optimized protocol for obtaining sufcient numbers of sand fy females fed on a second blood meal, and we described some physiological and parasitological aspects of the second gonotrophic cycle which might infuence the vectorial competence of sand fies.
INTRODUCTION
Visceral leishmaniasis (VL) is a severe chronic disease caused by protists belonging to the Leishmania donovani complex . Currently, the estimated incidence of VL is 0.2 to 0.4 million cases per year with over 90% occurring in India, Bangladesh, Sudan, South Sudan, Ethiopia, and Brazil . In the Americas, the phlebotomine sand fy utzomyia
longipalpis is the main vector of VL . Both male and female L. longipalpis adults, afer emergence from pupae, feed on sugars from plants and aphids in a regular basis, but only adult females are hematophagous, feeding on a wide .range of vertebrates as dogs, chickens, humans, and horses, among others
Year: ۲۰۱۸
Publisher : HINDAWI
By : C. S. Moraes, K. Aguiar-Martins, S. G. Costa, P. A. Bates , R. J. Dillon, and F. A. Genta
File Information: English Language/ 11 Page / size: 2.49 MB
سال : ۱۳۹۶
ناشر : HINDAWI
کاری از : C. S. Moraes، K. Aguiar-Martins، S. G. Costa، P. A. Bates، R. J. Dillon، F. A. Genta
اطلاعات فایل : زبان انگلیسی / 11 صفحه / حجم : MB 2.49
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