توضیحات
ABSTRACT
Natural plant extracts contain a variety of phenolic compounds which are assigned various biological activities. Our work aimsto make a quantitative and qualitative characterization of the Zest (ZL) and the Flesh (FL) of lemon (Citrus limon), to valorize the pharmacological uses of lemon, by evaluating in vitro activities (DPPH, free radical scavenging and reducing power). Te antibacterial, antifungal, and antiproliferative activities were sought in the ability of Citrus limon extracts to protect DNA and protein. We found that the ZL contains high amounts of phenolics responsible for the important antioxidant properties of the extract. However, the FL is richer in flavonoids than the ZL. Te FL extract was also found to be more effective than the ZL in protecting plasmid DNA against the strand breakage induced by hydroxyl radicals. We also concluded that the FL extract exhibited potent antibacterial activity unlike ZL. Analysis by LC/MS-MS identifed 6 compounds (Caffeoyl N-Tryptophan, Hydroxycinnamoyl-Oglucoside acid, Vicenin 2, Eriocitrin, Kaempferol-3-O- rutinoside, and Quercetin-3-rutinoside). Tese preliminary results showed that Citrus limon has antibacterial and antioxidant activity in vitro. It would be interesting to conduct further studies to evaluate the in vivo potential in an animal model.
INTRODUCTION
In recent years, many diseases have appeared and are mainly due to “oxidative stress” which is the result of an imbalance between the formations or not of prooxidants . Indeed, reactive oxygen species (ROS) are reactive molecules due to the presence of unpaired electrons such as superoxide anion radicals (O2∙-), hydroxyl radicals (OH∙), hydroperoxyl radicals (HOO∙), peroxyl (ROO∙), and also nonradical species such as hydrogen peroxide (H2O2), ozone (O3), and singlet oxygen (1O∙2) . Oxidative stress is caused by the presence of free radicals that upset stability by electronic pairing with several biological macromolecules such as proteins, lipids, and DNA
and cause signifcant damage to the basic structures of the body (proteins, lipid, and DNA) . Evidences that ROS accumulation in biological systems causes oxidative tissue damage and affects cellular integrity and function are tangible.
Year: ۲۰۱۹
Publisher : HINDAWE
By : Mohamed Makni , Raoua Jemai , Walid Kriaa, Yassine Chtourou, and Hamadi Fetoui
File Information: English Language/ 11 Page / size: 1.29MB
سال : ۱۳۹۷
ناشر : HINDAWE
کاری از : محمد مکنی، راوا یمامی، ولید کریا، یاسین چتروو و حمدی فتوی
اطلاعات فایل : زبان انگلیسی / 11 صفحه / حجم : MB 1.29
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