توضیحات
ABSTRACT
Tuberculosis meningitis (TBM) is a prevalent form of extra-pulmonary tuberculosis that causes substantial morbidity and mortality. Diagnosis of TBM is difficult because of the limited sensitivity of existing laboratory techniques. A metabolomics approach can be used to investigate the sets of metabolites of both bacteria and host, and has been used to clarify the mechanisms underlying disease development, and identify metabolic changes, leadings to improved methods for diagnosis, treatment, and prognostication. Mass spectrometry (MS) is a major analysis platform used in metabolomics, and MS-based metabolomics provides wide metabolite coverage, because of its high sensitivity, and is useful for the investigation of Mycobacterium tuberculosis (Mtb) and related diseases. It has been used to investigate TBM diagnosis; however, the processes involved in the MS-based metabolomics approach are complex and flexible, and often consist of several steps, and small changes in the methods used can have a huge impact on the final results. Here, the process of MS-based metabolomics is summarized and its applications in Mtb and Mtb-related diseases discussed. Moreover, the current status of TBM metabolomics is described.
INTRODUCTION
Tuberculosis meningitis (TBM) is an infectious disease of the nervous system caused by Mycobacterium tuberculosis (Mtb). TBM accounts for nearly 5% of all cases of extra-pulmonary tuberculosis , and it is associated with the highest rates of morbidity and mortality among all forms of tuberculosis . Outcomes of patients often include substantial neurological sequelae or death . Early diagnosis and initiation of appropriate treatment at an optimal dose can greatly improve the clinical outcomes of TBM ; however, it is difficult to make a definitive diagnosis of TBM, because of its variable and nonspecific clinical presentation, and the low sensitivity of current diagnostic laboratory tests. Hence, there is an urgent need for diagnostic variables that can accurately identify TBM. Metabolomics, an emerging science of the omics era, provides a new tool for the identification of novel diagnostic markers of TBM. This methodology is used to identify and quantify low-molecular-weight metabolites, and produces metabolite profiles which reflect the state of a given biological system .
Year: ۲۰۱۸
Publisher : ELSEVIER
By : Peixu Zhang, Weiguanliu Zhang, Yue Lang, Yan Qu, Fengna Chu,Jiafeng Chen, Li Cui
File Information: English Language/ 31 Page / size: 516 KB
سال : ۱۳۹۶
ناشر : ELSEVIER
کاری از : Peixu ژانگ، Weiguanliu ژانگ، یو Lang، یان کو، Fengna چو، Jiafeng چن، لی Cui
اطلاعات فایل : زبان انگلیسی / 31 صفحه / حجم : KB 516
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