Genetic characterization and modification-taliem-ir

Genetic characterization and modification of a bioethanol-producing yeast strain

10,000 تومان

Yeast Saccharomyces cerevisiae strains isolated from different sources generally show extensive genetic and phenotypic diversity. Understanding how genomic variations influence phenotypes is important for developing strategies with improved economic traits. The diploid S. cerevisiae strain NY1308 is used for cellulosic bioethanol production. Whole genome sequencing identified an extensive amount of single nucleotide variations and small insertions/deletions in the genome of NY1308 compared with the S288c genome. Gene annotation of the  assembled NY1308 genome showed that 43 unique genes are absent in the S288c genome. Phylogenetic analysis suggested most of the unique genes were obtained through horizontal gene transfer from other species. RNA-Seq revealed that some unique genes were not functional in NY1308 due to unidentified intron sequences. During bioethanol fermentation, NY1308 tends to flocculate when certain inhibitors (derived from the pretreatment of cellulosic feedstock) are present in the fermentation medium. qRT-PCR and genetic manipulation confirmed that the novel gene, NYn43, contributed to the flocculation ability of NY1308. Deletion of NYn43 resulted in a faster fermentation rate for NY1308. This work disclosed the genetic characterization of a bioethanol-producing S. cerevisiae strain and provided a useful paradigm showing how the genetic diversity of the yeast population would facilitate the personalized development of desirable traits.

 

دسته: , برچسب: , , , , ,

توضیحات

ABSTRACT

Yeast Saccharomyces cerevisiae strains isolated from different sources generally show extensive genetic and phenotypic diversity. Understanding how genomic variations influence phenotypes is important for developing strategies with improved economic traits. The diploid S. cerevisiae strain NY1308 is used for cellulosic bioethanol production. Whole genome sequencing identified an extensive amount of single nucleotide variations and small insertions/deletions in the genome of NY1308 compared with the S288c genome. Gene annotation of the  assembled NY1308 genome showed that 43 unique genes are absent in the S288c genome. Phylogenetic analysis suggested most of the unique genes were obtained through horizontal gene transfer from other species. RNA-Seq revealed that some unique genes were not functional in NY1308 due to unidentified intron sequences. During bioethanol fermentation, NY1308 tends to flocculate when certain inhibitors (derived from the pretreatment of cellulosic feedstock) are present in the fermentation medium. qRT-PCR and genetic manipulation confirmed that the novel gene, NYn43, contributed to the flocculation ability of NY1308. Deletion of NYn43 resulted in a faster fermentation rate for NY1308. This work disclosed the genetic characterization of a bioethanol-producing S. cerevisiae strain and provided a useful paradigm showing how the genetic diversity of the yeast population would facilitate the personalized development of desirable traits.

 

INTRODUCTION

 Yeast Saccharomyces cerevisiae is one of the most useful microorganisms in biological fermentation fields, such as winemaking, baking, brewing, and bioethanol production. The bioconversion of cellulosic biomass to ethanol is a promising technology to address fossil fuels shortages and greenhouse gas over-emission (Koutinas et al. 2016). To release the sugars that are trapped inside the cross-linking structure of cellulose, pretreatment processes (such steam explosion and dilute acid treatment) are always required prior to the enzymatic hydrolysis (Jönsson et al. 2013; Silveira et al. 2015).

Year: ۲۰۱۸

Publisher : SPRINGER

 By : Ke Zhang & Ya-Nan Di & Lei Qi & Yang Sui & Ting-Yu Wang & Li Fan & Zhen-Mei Lv & Xue-Chang Wu & Pin-Mei Wang & Dao-Qiong Zheng 

File Information: English Language/ 11 Page / size: 622 KB

Download

سال : ۱۳۹۶

ناشر : SPRINGER

کاری از : کی ژانگ و یان نوی دی و لی کی و یانگ سوئی و تینگ یو وانگ و لی فن و ژن مای لو و Xue-چانگ وو و پین مینی وانگ و دائو چیانگ ژنگ

اطلاعات فایل : زبان انگلیسی / 11 صفحه / حجم : KB 622

لینک دانلود      

 

نقد و بررسی‌ها

هنوز بررسی‌ای ثبت نشده است.

اولین کسی باشید که دیدگاهی می نویسد “Genetic characterization and modification of a bioethanol-producing yeast strain”

محصولات مرتبط

Using Metabolomics to Investigate Biomarkers of Drug AddictionUsing Metabolomics to Investigate Biomarkers-taliem-irDeepARG a deep learning approach for predicting-taliem-irDeepARG: a deep learning approach for predicting antibiotic resistance genes...