Effect of light and natural ventilation systems on the growth parameters and carvacrol content in the in vitro cultures of Plectranthus amboinicus (Lour.) Spreng

توضیحات

ABSTRACT

The aim of the current study is to investigate the influence of light intensity, quality of light and alternative membrane sytems on the growth and headspace-GC/ MS chemical analysis of Plectranthus amboinicus cultivated in vitro. Nodal segments were grown under light intensities (26, 51, 69, 94 and 130 µmol m-2 s-1) provided by cool-white fluorescent lamps. Apical segments were grown under light-emitting diodes blue; red; 1 blue/2.5 red; 2.5 blue/1 red; 1 blue/1 red and white fluorescent lamps. Apical and nodal segments were grown under alternative membrane and membrane-free systems. One, two or four PTFE membranes were used on the lid of the culture  vessel.The membranes provided natural ventilation and worked as flters. The results have shown signifcant differences in the growth and carvacrol content, as well as in the content of carvacrol precursors (γ-terpinene and p-cymene) in different treatments. Among all tested light intensities, the significant increase in the dry weight and in the carvacrol content of plantlets derived from the nodal segments was recorded at 69 µmol m-2 s-1. The monochromatic red led to greater shoot length and higher dry weight in plantlets derived from the apical segments, as well as to carvacrol accumulation greater than that provided by the fluorescent lamps. The culture vessel enclosure by one and two membranes led to higher dry weight in plantlets derived from the apical and nodal segments, respectively. They also showed higher carvacrol content. Thus, it is possible optimizing the rowth .and carvacrol content in P. amboinicus  ultivated in vitro by adjusting these environmental parameters

INTRODUCTION

Plectranthus amboinicus (Lour.) Spreng belongs to the family Lamiaceae. From an agricultural standpoint, it has
great medicinal potential, but is poorly studied (Arumugam et al. 2016). Recent studies have reported the species activity against breast cancer (Hasibuan et al. 2014). Plectranthus amboinicus essential oil is rich in thymol and carvacrol (Khare et al. 2011). These compounds besides having medicinal effect are used as natural preservative in the food industry (Trivellini et al. 2016). In addition, the species has environmental signifcance and can be used in the rhizofltration of lead-contaminated water (Ignatius et al. 2014).

Year: ۲۰۱۸

Publisher : SPRINGER

 By : Sâmia Torres Silva, Suzan Kelly Vilela Bertolucci,Samuel Henrique Braga da Cunha, Luiz Eduardo Santos Lazzarini,Marília Claudiano Tavares. José Eduardo Brasil Pereira Pinto

File Information: English Language/ 10 Page / size: 1.01 MB

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سال : ۱۳۹۶

ناشر : SPRINGER

کاری از : سمیرا تورس سیلوا، سوزان کلی ویلا بتولوچی، ساموئل هنریو براگا داو کانا، لوئیز ادواردو سانتوس لازارینی، مارلیا کلادیاو توارس. خوزه ادوارد برزیل پیریرا پینتو

اطلاعات فایل : زبان انگلیسی / 10 صفحه / حجم : MB 1.01

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